Expression of crylac in transgenic tobacco plants was assayed with elisa . the results showed that pinll signal peptide sequence enhanced the expression of crylac protein in transgenic tobacco 熒光顯微觀察到gfp在植物細胞間隙高效表達, westernblot結(jié)果顯示crylac蛋白也在植物細胞間隙表達。
Gfp gene was also fused behind the signal peptide sequence to construct plasmid p3301ubisiggfp and transformed to tobacco . the results of fluorescent detection showed gfp localization in the apoplast Elisa結(jié)果顯示,馬鈴薯蛋白酶抑制劑基因的信號肽序列使crylac基因在轉(zhuǎn)基因煙草中的表達量顯著提高。
We generated a recombinant eukaryotic gene expressing vector harboring a full - length hgh gene , 2 . 4 kb genomic dna with four introns and the signal peptide sequence cloned to the eukaryotic gene expressing vector pcdna3 . 0 我們直接將含有4個內(nèi)含子和自身信號肽的2 . 4kb全長人生長激素基因直接克隆至真核表達載體pcdna3 . 0 ,然后利用脂質(zhì)體轉(zhuǎn)染家蠶bmn細胞,瞬時表達hgh 。
To allow secretion of the crylac protein into the intercellular space , potato proteinase inhibitor ii ( pinll ) signal peptide sequence was n - terminally fused to the crylac coding region to construct plasmid p3301ubisigac 運用pcr技術(shù)克隆了馬鈴薯蛋白酶抑制劑基因的信號肽序列,并將其分別連到crylac 、 gfp基因的5 ’端,構(gòu)建植物轉(zhuǎn)化載體p3301ubisigac和p3301ubisiggfp 。
In order to explore transgenic plants for production of recombinant scfv specific for presl ( 20 - 47 ) , nicotiana tabacum was transformed with a gene encoding anti - presl of hepatitis b surface antigen scfv and bearing an / / - terminal endoplasmic reticulum protein signal peptide sequence 在用煙草作為生物反應器時,分別將該單鏈抗體靶向細胞質(zhì)和內(nèi)質(zhì)網(wǎng)。經(jīng)westernblot分析,靶向細胞質(zhì)中表達時,可溶單鏈抗體最高占總的可溶蛋白的0 . 06 。